| 1. | The combination of rh - cm with mitotically inactive mef feeder layer , was the best culture system
其中以mef作饲养层，添加rh - cm培养基一组效果最好。 |
| 2. | It was found that mef by treatment with mitomycin c has no significant difference as feeder layer as with y irradiation
研究发现采用丝裂霉素c或者射线处理mef作为饲养层没有明显的差异。 |
| 3. | Effects of mouse embryonic fibroblast feeder layers and combined of mouse embryonic fibroblast and collagen of rat tail on adhesion of cultured cells from schistosoma japonicum
鼠胚胎成纤维细胞饲养层及与鼠尾胶联合促日本血吸虫培养细胞贴壁作用的研究 |
| 4. | Mef were inactivated by treatment with 10ug / ml mitomycin c for 2 . 5 - 3 hours or y irradiation 50gy / 75gy dose and grew in dmem as feeder layer to help mouse es cells and human pgcs
采用10 g ml的丝裂霉素c或剂量为50gy和75gy的射线抑制mef有丝分裂，制作用于培养小鼠es细胞和人pgcs的饲养层。 |
| 5. | Eg cells of the 2th and 4th passage were akp ( alkaline phosphate ) positive . when cultured on degenerated feeder layers or in suspension , eg c ells formed embryoid bodies ( ebs ) in vitro
当eg细胞脱离饲养层悬浮培养，或在衰老的饲养层上延迟培养时，发现eg细胞或单个存在，或聚集成团，形成类似于早期胚胎的囊状胚体结构 |
| 6. | With the increase of passage , the total of colonies decreased , but the number of classical nest - like or island - like eg cell colonies relatively increased . the growth of eg cells was dependent on the feeder layer
传代培养的eg细胞对饲养层有依赖性， icr品系小鼠pgc细胞和由其传代后获得的eg细胞呈明显的群聚性，以单个存在状态的较少 |
| 7. | The clonies of es cells were flatter on the mitomycin c - inhibited sto fibroblasts , not easily differentiated from the feeder layer cells surround them , than that on the mef in dmem supplemented 15 % fbs . 5
以sto细胞作饲养层，添加15 fbs ，在倒置显微镜下观察， es - d3细胞不易与周围的sto饲养层细胞区别，形成的集落较平坦，生长状态不如mef饲养层好。 |
| 8. | Result : 1 ) dmem : f12 would enhance the proliferation of hpfl , especially present with a concentration of 20ng / ml eof ; pref feeder layer can defer the differentiation of hpfl in vitro , but collagen - vi and gelatin can not do
研究结果： 1 ）含有egf的dmem ： fiz对于hpfl的体外增殖明显优于其它培养液；在不同的培养基质中， pref饲养层优于明胶和胶原，可促进hpfl的生长，并能延缓hpfl在体外的分化。 |
| 9. | If alb + ck - 19 - cells would be induced into alb + ck - 19 + cells , we can prove that hpfl contain bipotential stem cells . 3 ) primary hpfl seeded on pref feeder layer were collected and engrafted into the spleen of sced mice to explore the propagation of hpfl in vivo
3 ）收集在原代大鼠成纤维细胞（ pref ）饲养层上培养的原代hpfl ，注人scto鼠脾脏，观察hpfl植人体内的增殖发育情况）将分离的大鼠ed13 |
| 10. | We also investigated the factors including feeder layer cells , growth factors and complements , which may affect the cultivation and differentiation of these cells in vitro . the main contents as follows : ( 1 ) isolation and culture of mouse embryonic fibroblast ( pmef ) and preparation the feeder layers
主要内容包括： ( 1 )小鼠胚胎成纤维细胞( mef )的分离培养和饲养层细胞的制作以妊娠13 . 5天或14 16天的小鼠为材料，建立了mef分离培养体系。 |
